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KMID : 0545119920020030166
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Journal of Microbiology and Biotechnology
1992 Volume.2 No. 3 p.166 ~ p.173
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Effects of Environmental Conditions on Expression of Bacillus subtilis α-Amylase in Recombinant Escherichia coli
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Shin, Pyong K.
Nam, Seung H./Seo, Jin Ho
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Abstract
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The expression of Bacillus subtilis α-amylase from the phoA-amyE fusion gene in recombinant E. coil was investigated under various environmental conditions. The overexpression of cloned α-amylase caused retardations in cell growth and synthesis of alkaline phosphatase (AP) from the chromosomal phoA gene. The change of culture temperature from 37℃ to 30℃ increased the specific activities of both α-amylase and β-lactamase by six and two times, respectively, whereas the AP activity remained unchanged. The experiments with chlorampenicol (a translation inhibitor) suggested the enhancement of α-amylase activity at 30℃, and this was partly due to the stability of α-amylase itself. The further decrease of the temperature to 25℃ slowed down both the cell growth and cloned-gene expression rate. The α-amylase activity showed a maximum at pH of 7.4 while alkaline phosphatase was most effectively produced at pH of 8.3.
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